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Glyphic Biotechnologies is developing a massively parallel, single-molecule proteome sequencing platform (ProSE) to transform life science discovery. The company has raised over $80M in venture funding and grant support to advance next-generation proteome sequencing.
You will join the Assay Integration team as a hands-on Scientist responsible for executing, optimizing, and troubleshooting sample preparation workflows across Glyphic's proteome sequencing platform and complementary genomic and proteomic assays. Reporting to the Director of Assay Integration, you will work with diverse biological inputs—cultured cells, fixed cells, tissue sections, blood, and key opinion leader samples—to generate high-quality data supporting platform benchmarking and multi-omic strategy.
Key responsibilities include:
**Sample Preparation & Assay Execution:**
- Evaluate and process incoming biological samples, assessing sample-specific constraints and analytical requirements
- Extract and quantify nucleic acids and proteins using established methods across diverse matrices
- Apply and adapt enrichment, depletion, and dynamic-range management protocols to optimize sequencing yield and target detection
- Perform core molecular biology reactions: buffer preparation, reagent formulation, enzymatic reactions, gel electrophoresis, PCR/qPCR, hybridization assays, and amplification workflows
- Generate sequencing-ready outputs: NGS libraries for DNA-seq, RNA-seq, and ribosome profiling; processed samples for ProSE and mass spectrometry
- Support generation and sourcing of standard reference materials using cell culture techniques
**Assay Optimization & Data Analysis:**
- Test protocol variables (reagent concentrations, incubation times, temperature, wash conditions, timing) to optimize assay performance
- Generate and analyze experimental data, evaluating performance using signal intensity, background, reproducibility, specificity, sensitivity, and robustness metrics
- Analyze large-scale proteomic and genomic datasets using quantitative and statistical approaches
- Propose and apply in-process controls and analytical tools to understand factors driving sequencing efficiency
- Collaborate with proteomics, assay development, chemistry, and data science teams to design experiments and guide project strategy
**Required Qualifications:**
- BS/MS/PhD in biochemistry, chemical biology, molecular biology, or equivalent field with 4+/2+/0+ years of relevant experience respectively
- Hands-on experience executing sample preparation protocols across proteomic and nucleic acid platforms
- Demonstrated skill preparing diverse biological sample types (cell lines, tissue, blood) for proteomic and genomic analysis
- Proficiency with NGS library preparation (DNA-seq, RNA-seq, ribosome profiling): nucleic acid extraction, quantification, amplification, QC
- Track record applying sample preparation and enrichment strategies
- Comfort troubleshooting multi-step protocols and adapting established methods to new sample types
- Ability to communicate results clearly across multidisciplinary teams
- Experience in high-throughput environments preferred
This is a full-time, exempt, in-person position based in Berkeley, CA.
